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In order to evaluate the suitability of tea (〖WTBX〗Camellia sinensis〖WTBZ〗) cultivation in the regions south of the Yangtze River, with the climate, topography, and soil factors taken into consideration, the article selects the annual average temperature, the annual accumulated temperature above 10 ℃, the frequency of extremely low temperature below -13 ℃, the average humidity from April to October, slope, aspect, altitude, soil pH, and soil texture to evaluate the comprehensive suitability in the regions south of the Yangtze River. The weights of suitability evaluation indexes are calculated by the AHP entropy method. Combined with the landuse map and the status of tea cultivation, the evaluation model of suitability of tea cultivation is established by the fine grid calculation. The suitability of tea cultivation is divided into 3 classes: suitable, subsuitable and nonsuitable regions. The results show that the areas of suitable, subsuitable and nonsuitable regions for tea cultivation are 3175183 km2，4103433 km2, and 1480645 km2, which accounted for 3625%, 4685% and 169% of the total evaluation area, respectively. Using the spatial distribution of tea cultivation of Songyang to validate and evaluate the comprehensive suitability regionalization of tea cultivation, it is demonstrated that the results of the model are identical with the spatial distribution of the status of tea cultivation. The evaluation results provide a scientific basis for the optimized layout of tea tree cultivation.