The negative accumulated temperature is an ecological indicator of heat resources in agriculture production in winter. In order to accelerate the agriculture development in frost season and give full play to the land resources, it is essential to find out the geographical distribution characteristics of negative accumulated temperature. Based on the daily mean air temperature of 48 meteorological stations in Liaoning Province from 1961 to 1990, the negative accumulated temperatures for all the stations and the geographical distribution characteristics were statistically calculated and analyzed. The multi-year calculation models of accumulated temperature average using latitude, longitude and altitude by means of the small grid method were developed, and the digital pictures of negative accumulated temperature can be drawn on the ARCGIS platform to improve the analysis precision.